Moscow Institute of Physics and Technology
3 ARTICLES PUBLISHED IN JoVE
Biochemistry
Imaging of Extracellular Vesicles by Atomic Force Microscopy
Mikhail Skliar 1,2, Vasiliy S. Chernyshev 3,4
1Department of Chemical Engineering, University of Utah, 2The Nano Institute of Utah, University of Utah, 3Center for Photonics and Quantum Materials, Skolkovo Institute of Science and Technology, 4Biopharmaceutical Cluster 'Northern', Moscow Institute of Physics and Technology
A step-by-step procedure is described for label-free immobilization of exosomes and extracellular vesicles from liquid samples and their imaging by atomic force microscopy (AFM). The AFM images are used to estimate the size of the vesicles in the solution and characterize other biophysical properties.
Immunology and Infection
Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
Ivan V. Maslov 1, Andrey O. Bogorodskiy 1, Mariia V. Pavelchenko 1, Ilia O. Zykov 1, Natalya I. Troyanova 2, Valentin I. Borshchevskiy 1,3, Marina A. Shevchenko 2
1Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 2Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 3Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich
We describe the method for quantitative analysis of the distribution of Aspergillus fumigatus conidia (3 µm in size) in the airways of mice. The method also can be used for the analysis of microparticles and nanoparticle agglomerate distribution in the airways in various pathological condition models.
Biochemistry
Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
Nadezhda Podoplelova 1,2, Polina Soloveva 1,4, Andrei Garzon Dasgupta 1,2,3, Aleksandra Filkova 1,2, Mikhail Panteleev 1,2,3,4
1Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, 2National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, 3Faculty of Physics, Lomonosov Moscow State University, 4Faculty of Biological and Medical Physics, Moscow Institute of Physics and Technology
Here, we describe a set of methods for characterizing the interaction of proteins with membranes of cells or microvesicles.
