Aptagen LLC
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
Tran Thi Thanh Thoa 1, Albert M. Liao 1, G. Thomas Caltagirone 1
1Aptagen LLC
The present protocol describes the use of Isothermal Titration Calorimetry (ITC) to analyze the association and dissociation kinetics of the binding between a DNA aptamer and tetracycline, including sample preparation, running standards and samples, and interpreting the resulting data.
Bioengineering
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Albert M. Liao 1, Tran Thi Thanh Thoa 1, G. Thomas Caltagirone 1
1Aptagen LLC
The present protocol demonstrates the use of a fast, 3-stage, aptamer-based exponential amplification assay to detect targets. Sample preparation, signal amplification, and color development are covered to implement this system to recognize the presence of theophylline over that of caffeine.
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