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University of Sao Paulo

9 ARTICLES PUBLISHED IN JoVE

Medicine

Vascular Occlusion Training for Inclusion Body Myositis: A Novel Therapeutic Approach

Bruno Gualano^1,2, Carlos Ugrinowitsch¹, Manoel Neves Jr.², Fernanda R. Lima², Ana Lúcia S. Pinto², Gilberto Laurentino¹, Valmor A.A. Tricoli¹, Antonio H. Lancha Jr.¹, Hamilton Roschel^1,2

¹School of Physical Education and Sport, University of São Paulo, ²Division of Rheumatology, School of Medicine, University of São Paulo

The present article presents the details pertaining to the application of resistance training associated to vascular occlusion in IBM patients.

Biology

Determining the Contribution of the Energy Systems During Exercise

Guilherme G. Artioli¹, Rômulo C. Bertuzzi², Hamilton Roschel^1,3, Sandro H. Mendes¹, Antonio H. Lancha Jr.¹, Emerson Franchini⁴

¹Laboratory of Applied Nutrition, School of Physical Education and Sport, University of Sao Paulo, ²Aerobic Performance Research Group, School of Physical Education and Sport, University of Sao Paulo, ³Laboratory of Neuromuscular Adaptations to Strength Training, School of Physical Education and Sport, University of Sao Paulo, ⁴Martial Arts and Combat Sports Research Group, School of Physical Education and Sport, University of Sao Paulo

This protocol allows researchers focused on exercise and sports sciences to determine the relative contribution of three different energy systems to the total energy expenditure during a large variety of exercises.

Immunology and Infection

Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

Amrita Sarkar¹, Yousuf A. Khan¹, Maria Fernanda Laranjeira-Silva¹, Norma W. Andrews¹, Bidyottam Mittra¹

¹Department of Cell Biology and Molecular Genetics, University of Maryland

All pathogenic Leishmania species reside and replicate inside macrophages of their vertebrate hosts. Here, we present a protocol to infect murine bone marrow-derived macrophages in culture with Leishmania, followed by precise quantification of intracellular growth kinetics. This method is useful for studying individual factors influencing host-pathogen interaction and Leishmania virulence.

Neuroscience

Real-time Video Projection in an MRI for Characterization of Neural Correlates Associated with Mirror Therapy for Phantom Limb Pain

Faddi G. Saleh Velez^1,2, Camila B. Pinto^1,3, Emma S. Bailin⁴, Marionna Münger¹, Andrew Ellison⁵, Beatriz T. Costa¹, David Crandell⁶, Nadia Bolognini^7,8, Lotfi B. Merabet⁴, Felipe Fregni¹

¹Laboratory of Neuromodulation & Center for Clinical Research Learning, Department of Physical Medicine and Rehabilitation, Harvard Medical School, Spaulding Rehabilitation Hospital, ²University of Chicago Medical Center, Department of Neurology, University of Chicago, ³Department of Neuroscience and Behavior, Psychology Institute, University of Sao Paulo, ⁴The Laboratory for Visual Neuroplasticity, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, ⁵Center for Biomedical Imaging, Department of Anatomy and Neurobiology, Boston University School of Medicine, ⁶Spaulding Rehabilitation Hospital, Harvard Medical School, ⁷Department of Psychology & Milan Center for Neuroscience, University of Milano-Bicocca, ⁸Neuropsychological Laboratory, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Istituto Auxologico Italiano

We present a novel combined behavioral and neuroimaging protocol employing real-time video projection for the purpose of characterizing the neural correlates associated with mirror therapy within the magnetic resonance imaging scanner environment in leg amputee subjects with phantom limb pain.

Immunology and Infection

In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice

Juliana Ide Aoki¹, Ahyun Hong¹, Ricardo Andrade Zampieri¹, Lucile Maria Floeter-Winter¹, Maria Fernanda Laranjeira-Silva¹

¹Department of Physiology, Institute of Bioscience, University of Sao Paulo

Here, we present a compiled protocol to evaluate the cutaneous infection of mice with Leishmania amazonensis. This is a reliable method for studying parasite virulence, allowing a systemic view of the vertebrate host response to the infection.

Biochemistry

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Felipe A. Carvalho¹, Moises R. A. Barros¹, Thierry P. F. Girotto¹, M. Griselda Perona¹, Carla C. Oliveira¹

¹Department of Biochemistry, Institute of Chemistry, University of Sao Paulo

Here, we describe the utilization of Grafix (Gradient Fixation), a glycerol gradient centrifugation in the presence of a crosslinker, to identify interactions between splicing factors that bind transiently to the spliceosome complex.

Immunology and Infection

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

Rafaela S. Fernandes¹, Gabriela D. Noske¹, Victor O. Gawriljuk¹, Ketllyn I. Z. de Oliveira¹, Andre S. Godoy¹, Nathalya C. M. R. Mesquita¹, Glaucius Oliva¹

¹São Carlos Institute of Physics, University of Sao Paulo

In this work, we describe the protocols used in replicon-based and viral enzyme-based assays to screen for inhibitors of Zika virus replication in a high-throughput screening format.

Genetics

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Natália Yumi Noronha^*1, Guilherme da Silva Rodrigues^*1, Marcela Augusta de Souza Pinhel¹, Jean-Baptiste Cazier^2,3, Lígia Moriguchi Watanabe¹, Albert Nobre Menezes⁴, Carlos Roberto Bueno⁵, Carolina Ferreira Nicoletti¹, Bruno Affonso Parenti de Oliveira¹, Isabelle Mello Schineider⁶, Isabella Harumi Yonehara Noma⁷, Igor Caetano Dias Alcarás⁸, Fernando Barbosa⁹, Carla Barbosa Nonino⁶

¹Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, ²Centre for Computational Biology, University of Birmingham, ³Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, ⁴Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine, The University of Edinburgh, ⁵Ribeirão Preto School of Physical Education and Sport, University of São Paulo, ⁶Health Sciences Department, Ribeirao Preto Medical School, University of Sao Paulo, ⁷Faculty of Pharmaceutical Sciences, University of São Paulo, ⁸Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, ⁹Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo

The present study describes the workflow to manage DNA methylation data obtained by microarray technologies. The protocol demonstrates steps from sample preparation to data analysis. All procedures are described in detail, and the video shows the significant steps.

Medicine

Randomized Controlled Trial to Study the Acute Effects of Strength Exercise on Insulin Sensitivity in Obese Adults

Luis Filipe Rocha Silva¹, Bruna Caroline Chaves Garcia², Zach A. Mang³, Fabiano Trigueiro Amorim⁴, Marco Fabrício Dias-Peixoto¹, Fernando Gripp¹, Valmor Tricoli⁵, Flávio de Castro Magalhães^1,4

¹Department of Physical Education, Federal University of the Jequitinhonha and Mucuri Valleys, ²Laboratory of Exercise Biology and Immunometabolism, Centro Integrado de Pós-Graduação e Pesquisa em Saúde, Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas, Federal University of the Jequitinhonha and Mucuri Valleys, ³Occupational Safety & Health, Los Alamos National Laboratory, ⁴Department of Health, Exercise, and Sports Sciences, University of New Mexico, ⁵School of Physical Education and Sport, University of Sao Paulo

This study describes a randomized controlled trial protocol aiming at assessing the acute effects of strength exercise volume on insulin sensitivity in obese individuals.