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Universidad de Chile

5 ARTICLES PUBLISHED IN JoVE

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Bioengineering

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo
Thilo L. Schenck 1, Myra N. Chávez 1, Alexandru P. Condurache 2, Ursula Hopfner 1, Farid Rezaeian 3, Hans-Günther Machens 1, José T. Egaña 1,4
1Department of Plastic Surgery and Hand Surgery, University Hospital rechts der Isar, Technische Universität München, 2Institute for Signal Processing, University of Lübeck, 3Department of Plastic Surgery and Hand Surgery, University Hospital Zürich, 4FONDAP Center for Genome Regulation, Facultad de Ciencias, Universidad de Chile

Vascularization is key to approaches in successful tissue engineering. Therefore, reliable technologies are required to evaluate the development of vascular networks in tissue-constructs. Here we present a simple and cost-effective method to visualize and quantify vascularization in vivo.

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Medicine

Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia
Yves Harder 1, Daniel Schmauss 1, Reto Wettstein 2, José T. Egaña 1, Fabian Weiss 1, Andrea Weinzierl 1, Anna Schuldt 1, Hans-Günther Machens 1, Michael D. Menger 3, Farid Rezaeian 4
1Department of Plastic Surgery and Hand Surgery, Klinikum rechts der Isar, Technische Universität München, 2Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital of Basel, 3Institute for Clinical and Experimental Surgery, University of Saarland, 4Division of Plastic and Hand Surgery, University Hospital Zurich

The window of the murine dorsal skinfold chamber presented visualizes a zone of acute persistent ischemia of a musculocutaneous flap. Intravital epi-fluorescence microscopy permits for direct and repetitive assessment of the microvasculature and quantification of hemodynamics. Morphologic and hemodynamic results can further be correlated with histological and molecular analyses.

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Biochemistry

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure
Aaron J. Paredes 1, Hilda M. Alfaro-Valdés 1, Christian A.M. Wilson 1
1Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile

This method allows selective staining and quantification of DNA in gels by soaking the gel in a SYBR Green I/Nitro Blue Tetrazolium solution and then exposing it to sunlight or a blue light source. This produces a visible precipitate and requires almost no equipment, making it ideal for field use.

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Behavior

Eye Tracking During Visually Situated Language Comprehension: Flexibility and Limitations in Uncovering Visual Context Effects
Camilo Rodriguez Ronderos 1, Katja Münster 1, Ernesto Guerra 2, Helene Kreysa 3, Alba Rodríguez 1,4, Julia Kröger 4, Thomas Kluth 4, Michele Burigo 4, Dato Abashidze 5, Eva Nunnemann 4, Pia Knoeferle 1,6,7
1Institute of German Studies and Linguistics, Humboldt-Universität zu Berlin, 2Center for Advanced Research in Education – CIAE, Universidad de Chile, 3Institute of Psychology, Friedrich Schiller University Jena, 4Cognitive Interaction Technology Excellence Cluster (CITEC), Faculty of Linguistics and Literature, Bielefeld University, 5Department of Education, Concordia University, 6Berlin School of Mind and Brain, 7Einstein Center for Neurosciences Berlin

The present article reviews an eye-tracking methodology for studies on language comprehension. To obtain reliable data, key steps of the protocol must be followed. Among these are the correct set-up of the eye tracker (e.g., ensuring good quality of the eye and head images) and accurate calibration.

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Neuroscience

Visualizing Monocarboxylates and Other Relevant Metabolites in the Ex Vivo Drosophila Larval Brain Using Genetically Encoded Sensors
Andrés González-Gutiérrez 1, Jorge Gaete 1, Andrés Esparza 1, Jorge Toledo 2, Andrés Köhler-Solis 1, Jimena Sierralta 1,3
1Biomedical Neuroscience Institute, Universidad de Chile, 2Advanced Scientific Equipment Network (REDECA), Faculty of Medicine, Universidad de Chile, 3Department of Neuroscience, Faculty of Medicine, Universidad de Chile

Here we present a protocol to visualize the transport of monocarboxylates, glucose, and ATP in glial cells and neurons using genetically encoded Förster resonance energy transfer-based sensors in an ex-vivo Drosophila larval brain preparation.

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