The DNA staining protocol has three general steps. The first is to put the gel in a solution of SYBR Green I and nitro blue tetrazolium, also known as NBT. The gel is then stirred for 25 minutes if it is a polyacrylamide gel and an hour if it is a
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This method allows selective staining and quantification of DNA in gels by soaking the gel in a SYBR Green I/Nitro Blue Tetrazolium solution and then exposing it to sunlight or a blue light source. This produces a visible precipitate and requires almost no equipment, making it ideal for field use.