Université Aix-Marseille
2 ARTICLES PUBLISHED IN JoVE
Biology
Cell Labeling and Injection in Developing Embryonic Mouse Hearts
Emilye Hiriart *1, Patrick van Vliet *2, Ralf J. Dirschinger 2, Sylvia M. Evans 2, Michel Puceat 1
1INSERM UMR-910, Aix-Marseille University, 2Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego
We describe a series of methods to inject dyes, DNA vectors, virus, and cells in order to monitor both cell fate and phenotype of endogenous and grafted cells derived from embryonic or pluripotent cells within mouse embryos at embryonic day (E)9.5 and later stages of development.
Developmental Biology
Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
Imen Jebeniani 1, Julia Leschik 2, Michel Puceat 1
1INSERM UMR-910, Faculté de Médecine, Université Aix-Marseille, 2Institute of Physiological Chemistry, University Medical Center, Johannes Gutenberg University
A fine tuning regulation of gene transcription underlies embryonic cell fate decision. Herein, we describe chromatin immunoprecipitation assays used to investigate epigenetic regulation of both cardiac differentiation of stem cells and cardiac development of mouse embryos.
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