Collection of E9.5 and E10.5 Embryos for Ex vivo Injection
3:10
Ex vivo DNA or Cell Injection Under a Stereomicroscope
4:35
Results: Embryonic Heart Cell Labeling and Injection
6:30
Conclusion
We describe a series of methods to inject dyes, DNA vectors, virus, and cells in order to monitor both cell fate and phenotype of endogenous and grafted cells derived from embryonic or pluripotent cells within mouse embryos at embryonic day (E)9.5 and later stages of development.