Accedi

Université Aix-Marseille

2 ARTICLES PUBLISHED IN JoVE

Biology

Cell Labeling and Injection in Developing Embryonic Mouse Hearts

Emilye Hiriart^*1, Patrick van Vliet^*2, Ralf J. Dirschinger², Sylvia M. Evans², Michel Puceat¹

¹INSERM UMR-910, Aix-Marseille University, ²Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego

We describe a series of methods to inject dyes, DNA vectors, virus, and cells in order to monitor both cell fate and phenotype of endogenous and grafted cells derived from embryonic or pluripotent cells within mouse embryos at embryonic day (E)9.5 and later stages of development.

Developmental Biology

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Imen Jebeniani¹, Julia Leschik², Michel Puceat¹

¹INSERM UMR-910, Faculté de Médecine, Université Aix-Marseille, ²Institute of Physiological Chemistry, University Medical Center, Johannes Gutenberg University

A fine tuning regulation of gene transcription underlies embryonic cell fate decision. Herein, we describe chromatin immunoprecipitation assays used to investigate epigenetic regulation of both cardiac differentiation of stem cells and cardiac development of mouse embryos.