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Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico

5 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
Giulia Ercoli 1, Gianluca Lopez 1,2, Camilla Ciapponi 1,3, Chiara Corti 1,2, Luca Despini 4, Donatella Gambini 5, Letterio Runza 1, Concetta Blundo 4, Amedeo Sciarra 1, Nicola Fusco 1,6
1Division of Pathology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 2School of Medicine, University of Milan, 3School of Biology, University of Pavia, 4Division of Breast Surgery, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, 5Division of Medical Oncology, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, 6Department of Biomedical, Surgical, and Dental Sciences, University of Milan

Heterogeneous distribution of HER2-positive cells can be observed in a subset of breast cancers and generates clinical dilemmas. Here, we introduce a reliable and cost-effective protocol to define, quantify, and compare HER2 intra-tumor genetic heterogeneity in a large series of heterogeneously processed breast cancers.

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Immunology and Infection

Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses
Fulvia Milena Cribiù *1, Claudia Burrello *2, Roberta Tacchi 1, Francesca Boggio 1, Dario Ricca 1, Flavio Caprioli 3,4, Stefano Ferrero 1, Federica Facciotti 2
1Pathology Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, 2Department of Experimental Oncology, European Institute of Oncology, 3Gastroenterology and Endoscopy Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, 4Department of Pathophysiology and Transplantation, Università degli Studi di Milano

Lack of standardization for murine tissue processing reduces the quality of murine histopathological analysis as compared to human specimens. Here, we present a protocol to perform histopathological examination of murine inflamed and uninflamed colonic tissues to show the feasibility of robotic systems routinely used for processing and embedding human samples.

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Medicine

Integrating Augmented Reality Tools in Breast Cancer Related Lymphedema Prognostication and Diagnosis
Marco Invernizzi 1, Letterio Runza 2, Alessandro De Sire 1, Lorenzo Lippi 1, Concetta Blundo 3, Donatella Gambini 4, Renzo Boldorini 5,6, Stefano Ferrero 2,7, Nicola Fusco 2,7
1Physical and Rehabilitative Medicine, Department of Health Sciences, University of Eastern Piedmont "A. Avogadro", 2Division of Pathology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 3Division of Breast Surgery, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 4Division of Medical Oncology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 5Division of Pathology, Maggiore della Carità Hospital, 6Department of Health Sciences, University of Eastern Piedmont "A. Avogadro", 7Department of Biomedical, Surgical, and Dental Sciences, University of Milan

Breast cancer related lymphedema is frequent in breast cancer survivors, but there are not widely employed guidelines for its diagnosis and quantification. Here, we introduce a reliable and cost-effective protocol to define, quantify, and compare upper limb volume in breast cancer patients.

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Medicine

Laparoscopic Oocyte Retrieval and Cryopreservation during Vaginoplasty for Treatment of Mayer-Rokitansky-Kuster-Hauser Syndrome
Valeria Stella Vanni *1,2, Alessandra Alteri *1,2, Lucia De Santis 1,2, Greta Chiara Cermisoni 1, Elisa Rabellotti 1, Diana Delprato 1, Marta Parma 1, Enrico Papaleo 1, Luigi Fedele 3, Massimo Candiani 1,2
1Obstetrics and Gynaecology Unit, IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University, 3Department of Obstetrics and Gynecology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico

A dedicated team can offer Mayer-Rokitansky-Kuster-Hauser patients the option to perform controlled ovarian stimulation and oocyte cryopreservation at the time of laparoscopic vaginoplasty.

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Cancer Research

Biobank for Translational Medicine: Standard Operating Procedures for Optimal Sample Management
Giuseppina Bonizzi *1, Maria Capra *1, Cristina Cassi 1, Giulio Taliento 1, Oriana Pala 2, Elham Sajjadi 2,3, Konstantinos Venetis 2,3, Mariia Ivanova 2, Massimo Monturano 4, Giuseppe Renne 2, Lorenzo Zattoni 1,3, Elena Guerini-Rocco 2,3, Giuseppe Viale 2,3, Roberto Orecchia 5, Nicola Fusco 1,2,3
1Biobank for Translational and Digital Medicine Unit, IEO, European Institute of Oncology IRCCS, 2Division of Pathology, IEO, European Institute of Oncology IRCCS, 3Department of Oncology and Hemato-Oncology, University of Milan, 4Patient Safety & Risk Management Service, IEO, European Institute of Oncology IRCCS, 5Scientific Directorate, IEO, European Institute of Oncology IRCCS

Biobanks are crucial resources for biomedical research and the Biobank for Translational and Digital Medicine Unit at the European Institute of Oncology is a model in this field. Here, we provide a detailed description of biobanks' standard operating procedures for the management of different types of human biological samples.

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