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Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico

5 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Giulia Ercoli¹, Gianluca Lopez^1,2, Camilla Ciapponi^1,3, Chiara Corti^1,2, Luca Despini⁴, Donatella Gambini⁵, Letterio Runza¹, Concetta Blundo⁴, Amedeo Sciarra¹, Nicola Fusco^1,6

¹Division of Pathology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, ²School of Medicine, University of Milan, ³School of Biology, University of Pavia, ⁴Division of Breast Surgery, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, ⁵Division of Medical Oncology, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, ⁶Department of Biomedical, Surgical, and Dental Sciences, University of Milan

Heterogeneous distribution of HER2-positive cells can be observed in a subset of breast cancers and generates clinical dilemmas. Here, we introduce a reliable and cost-effective protocol to define, quantify, and compare HER2 intra-tumor genetic heterogeneity in a large series of heterogeneously processed breast cancers.

Immunology and Infection

Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses

Fulvia Milena Cribiù^*1, Claudia Burrello^*2, Roberta Tacchi¹, Francesca Boggio¹, Dario Ricca¹, Flavio Caprioli^3,4, Stefano Ferrero¹, Federica Facciotti²

¹Pathology Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, ²Department of Experimental Oncology, European Institute of Oncology, ³Gastroenterology and Endoscopy Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, ⁴Department of Pathophysiology and Transplantation, Università degli Studi di Milano

Lack of standardization for murine tissue processing reduces the quality of murine histopathological analysis as compared to human specimens. Here, we present a protocol to perform histopathological examination of murine inflamed and uninflamed colonic tissues to show the feasibility of robotic systems routinely used for processing and embedding human samples.

Medicine

Integrating Augmented Reality Tools in Breast Cancer Related Lymphedema Prognostication and Diagnosis

Marco Invernizzi¹, Letterio Runza², Alessandro De Sire¹, Lorenzo Lippi¹, Concetta Blundo³, Donatella Gambini⁴, Renzo Boldorini^5,6, Stefano Ferrero^2,7, Nicola Fusco^2,7

¹Physical and Rehabilitative Medicine, Department of Health Sciences, University of Eastern Piedmont "A. Avogadro", ²Division of Pathology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, ³Division of Breast Surgery, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, ⁴Division of Medical Oncology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, ⁵Division of Pathology, Maggiore della Carità Hospital, ⁶Department of Health Sciences, University of Eastern Piedmont "A. Avogadro", ⁷Department of Biomedical, Surgical, and Dental Sciences, University of Milan

Breast cancer related lymphedema is frequent in breast cancer survivors, but there are not widely employed guidelines for its diagnosis and quantification. Here, we introduce a reliable and cost-effective protocol to define, quantify, and compare upper limb volume in breast cancer patients.

Medicine

Laparoscopic Oocyte Retrieval and Cryopreservation during Vaginoplasty for Treatment of Mayer-Rokitansky-Kuster-Hauser Syndrome

Valeria Stella Vanni^*1,2, Alessandra Alteri^*1,2, Lucia De Santis^1,2, Greta Chiara Cermisoni¹, Elisa Rabellotti¹, Diana Delprato¹, Marta Parma¹, Enrico Papaleo¹, Luigi Fedele³, Massimo Candiani^1,2

¹Obstetrics and Gynaecology Unit, IRCCS San Raffaele Scientific Institute, ²Vita-Salute San Raffaele University, ³Department of Obstetrics and Gynecology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico

A dedicated team can offer Mayer-Rokitansky-Kuster-Hauser patients the option to perform controlled ovarian stimulation and oocyte cryopreservation at the time of laparoscopic vaginoplasty.

Cancer Research

Biobank for Translational Medicine: Standard Operating Procedures for Optimal Sample Management

Giuseppina Bonizzi^*1, Maria Capra^*1, Cristina Cassi¹, Giulio Taliento¹, Oriana Pala², Elham Sajjadi^2,3, Konstantinos Venetis^2,3, Mariia Ivanova², Massimo Monturano⁴, Giuseppe Renne², Lorenzo Zattoni^1,3, Elena Guerini-Rocco^2,3, Giuseppe Viale^2,3, Roberto Orecchia⁵, Nicola Fusco^1,2,3

¹Biobank for Translational and Digital Medicine Unit, IEO, European Institute of Oncology IRCCS, ²Division of Pathology, IEO, European Institute of Oncology IRCCS, ³Department of Oncology and Hemato-Oncology, University of Milan, ⁴Patient Safety & Risk Management Service, IEO, European Institute of Oncology IRCCS, ⁵Scientific Directorate, IEO, European Institute of Oncology IRCCS

Biobanks are crucial resources for biomedical research and the Biobank for Translational and Digital Medicine Unit at the European Institute of Oncology is a model in this field. Here, we provide a detailed description of biobanks' standard operating procedures for the management of different types of human biological samples.