Four-plasmid Cotransfection Mediated by Lipofection
2:15
Susceptible Cell Seeding and Pseudotyped Viral Particle Quantification
4:01
Infectivity Assay
5:34
Results: Infectivity Assay of Pseudotyped Particles
6:58
Conclusion
Transcribir
This technique of producing influenza viral PP and determining its infectivity can simplify the research of influenza virus in a BSL-2 city. We normalized the PPS for the infections based on qRT-PCR data of PPS. Two glycoprotein expression plasmid
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This protocol describes an experimental process to produce high-titer infectious viral pseudotyped particles (pp) with envelope glycoproteins from two influenza A strains and how to determine their infectivity. This protocol is highly adaptable to develop pps of any other type of enveloped viruses with different envelope glycoproteins.