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Sir Mortimer B. Davis-Jewish General Hospital

4 ARTICLES PUBLISHED IN JoVE

Biology

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Yanning Wu¹, Shuo Wang¹, Chunying Li^1,2,3

¹Department of Biochemistry & Molecular Biology, Wayne State University School of Medicine, ²Cardiovascular Research Institute, Wayne State University School of Medicine, ³Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine

Cystic fibrosis transmembrane conductance regulator (CFTR), an epithelial chloride channel, has been reported to interact with various proteins and regulate important cellular processes; among them the CFTR PDZ motif-mediated interactions have been well documented. This protocol describes methods we developed to assemble a PDZ-dependent CFTR macromolecular signaling complex in vitro.

Behavior

Creating Dynamic Images of Short-lived Dopamine Fluctuations with lp-ntPET: Dopamine Movies of Cigarette Smoking

Evan D. Morris^1,2,3,4, Su Jin Kim^1,3, Jenna M. Sullivan^1,3,4, Shuo Wang^3,4, Marc D. Normandin⁵, Cristian C. Constantinescu⁶, Kelly P. Cosgrove^1,2,3

¹Diagnostic Radiology, Yale University, ²Psychiatry, Yale University, ³Yale PET Center, Yale University, ⁴Biomedical Engineering, Yale University, ⁵Nuclear Medicine, Massachusetts General Hospital, ⁶Radiological Sciences, University of California, Irvine

We present a novel PET imaging approach for capturing dopamine fluctuations induced by cigarette smoking. Subjects smoke in the PET scanner. Dynamic PET images are modeled voxel-by-voxel in time by lp-ntPET, which includes a time-varying dopamine term. The results are 'movies' of dopamine fluctuations in the striatum during smoking.

Behavior

Simultaneous Eye Tracking and Single-Neuron Recordings in Human Epilepsy Patients

Shuo Wang¹, Nand Chandravadia², Adam N. Mamelak², Ueli Rutishauser^2,3,4

¹Department of Chemical and Biomedical Engineering, and Rockefeller Neuroscience Institute, West Virginia University, ²Departments of Neurosurgery and Neurology, Cedars-Sinai Medical Center, ³Center for Neural Science and Medicine, Department of Biomedical Sciences, Cedars-Sinai Medical Center, ⁴Division of Biology and Biological Engineering, California Institute of Technology

We describe a method to conduct single-neuron recordings with simultaneous eye tracking in humans. We demonstrate the utility of this method and illustrate how we used this approach to obtain neurons in the human medial temporal lobe that encode targets of a visual search.

Cancer Research

Detection of Lung Tumor Progression in Mice by Ultrasound Imaging

Nour Ghaddar^1,2, Shuo Wang¹, Véronique Michaud¹, Urszula Kazimierczak^1,3, Nicolas Ah-son¹, Antonis E. Koromilas^1,4

¹Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, ²Division of Experimental Medicine, Faculty of Medicine, McGill University, ³Department of Cancer Immunology, Chair of Medical Biotechnology, Poznan University of Medical Sciences, ⁴Gerald Bronfman Department of Oncology, Faculty of Medicine, McGill University

This protocol describes the steps taken to induce KRAS lung tumors in mice as well as the quantification of formed tumors by ultrasound imaging. Small tumors are visualized in early timepoints as B-lines. At later timepoints, relative tumor volume measurements are achieved by the measurement tool in the ultrasound software.