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Federal University of ABC

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

3D Modeling of Dendritic Spines with Synaptic Plasticity

Gabriela Antunes^*1, Fabio M. Simoes de Souza^*1

¹Center for Mathematics, Computation and Cognition, Federal University of ABC

The protocol develops a three-dimensional (3D) model of a dendritic segment with dendritic spines for modeling synaptic plasticity. The constructed mesh can be used for computational modeling of AMPA receptor trafficking in the long-term synaptic plasticity using the software program Blender with CellBlender and MCell.

JoVE Core

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells

Rafael Badain¹, Daniel S. C. Damineli^1,2, Maria Teresa Portes³, José Feijó⁴, Stefano Buratti⁵, Giorgia Tortora⁶, Hugo Neves de Oliveira¹, Roberto M. Cesar Jr¹

¹Institute of Mathematics and Statistics, University of São Paulo, ²Center for Mathematics, Computing and Cognition, Federal University of ABC, ³Department of Botany, Institute of Biosciences, University of São Paulo, ⁴Cell Biology and Molecular Genetics Department, University of Maryland, ⁵Department of Biosciences, University of Milan, ⁶Department of Physics, Politecnico di Milano

Current methods for analyzing the intracellular dynamics of polarized single cells are often manual and lack standardization. This manuscript introduces a novel image analysis pipeline for automating midline extraction of single polarized cells and quantifying spatiotemporal behavior from time lapses in a user-friendly online interface.