Federal University of ABC
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
3D Modeling of Dendritic Spines with Synaptic Plasticity
Gabriela Antunes *1, Fabio M. Simoes de Souza *1
1Center for Mathematics, Computation and Cognition, Federal University of ABC
The protocol develops a three-dimensional (3D) model of a dendritic segment with dendritic spines for modeling synaptic plasticity. The constructed mesh can be used for computational modeling of AMPA receptor trafficking in the long-term synaptic plasticity using the software program Blender with CellBlender and MCell.
JoVE Core
AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
Rafael Badain 1, Daniel S. C. Damineli 1,2, Maria Teresa Portes 3, José Feijó 4, Stefano Buratti 5, Giorgia Tortora 6, Hugo Neves de Oliveira 1, Roberto M. Cesar Jr 1
1Institute of Mathematics and Statistics, University of São Paulo, 2Center for Mathematics, Computing and Cognition, Federal University of ABC, 3Department of Botany, Institute of Biosciences, University of São Paulo, 4Cell Biology and Molecular Genetics Department, University of Maryland, 5Department of Biosciences, University of Milan, 6Department of Physics, Politecnico di Milano
Current methods for analyzing the intracellular dynamics of polarized single cells are often manual and lack standardization. This manuscript introduces a novel image analysis pipeline for automating midline extraction of single polarized cells and quantifying spatiotemporal behavior from time lapses in a user-friendly online interface.
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