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Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund Berlin

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Maria Lucia Pigazzini^*1,2, Christian Gallrein^*1, Manuel Iburg^*1, Gabriele Kaminski Schierle³, Janine Kirstein^1,4

¹Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund Berlin, ²NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, ³Molecular Neuroscience Group, Department of Chemical Engineering and Biotechnology, University of Cambridge, ⁴Cell Biology, University of Bremen

Fluorescence lifetime imaging monitors, quantifies and distinguishes the aggregation tendencies of proteins in living, aging, and stressed C. elegans disease models.

Biology

In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2

Maria Lucia Pigazzini^1,2, Janine Kirstein^1,3

¹Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund, ²NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, ³Department of Biology and Chemistry, University of Bremen

Presented here is a protocol to monitor degradation of the protein huntingtin fused to the photoconvertible fluorophore Dendra2.