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Max Delbrueck Center for Molecular Medicine and Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Monitoring Dendritic Cell Migration using ¹⁹F / ¹H Magnetic Resonance Imaging

Helmar Waiczies^1,2, Martin Guenther^1,2, Julia Skodowski^1,2, Stefano Lepore^1,2, Andreas Pohlmann², Thoralf Niendorf^1,2, Sonia Waiczies^1,2

¹Experimental and Clinical Research Center, A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, ²Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine

Tracking of cells using MRI has gained remarkable attention in the past years. This protocol describes the labeling of dendritic cells with fluorine (¹⁹F)-rich particles, the in vivo application of these cells, and monitoring the extent of their migration to the draining lymph node with ¹⁹F/¹H MRI and ¹⁹F MRS.

JoVE Core

Cardiac Magnetic Resonance Imaging at 7 Tesla

Daniel Stäb^1,2, Aiman Al Najjar¹, Kieran O'Brien^1,3, Wendy Strugnell⁴, Jonathan Richer³, Jan Rieger⁵, Thoralf Niendorf⁵, Markus Barth¹

¹The Centre for Advanced Imaging, The University of Queensland, Brisbane, Australia, ²Department of Diagnostic and Interventional Radiology, University Clinic Würzburg, Würzburg, Germany, ³Siemens Healthcare Pty Ltd, Brisbane, Australia, ⁴Richard Slaughter Centre of Excellence in CVMRI, The Prince Charles Hospital, Brisbane, Australia, ⁵MRI.Tools GmbH, Berlin, Germany

The sensitivity gain inherent to ultrahigh field magnetic resonance holds promise for high spatial resolution imaging of the heart. Here, we describe a protocol customized for functional cardiovascular magnetic resonance (CMR) at 7 Tesla using an advanced multi-channel radio-frequency coil, magnetic field shimming and a triggering concept.

Medicine

Magnetic Resonance Imaging of Multiple Sclerosis at 7.0 Tesla

Sonia Waiczies^*1, Antje Els^*1, Joseph Kuchling^*2,3,4, Karin Markenroth Bloch⁵, Anna Pankowska^6,7, Helmar Waiczies⁸, Carl Herrmann¹, Claudia Chien^2,3, Carsten Finke^4,9, Friedemann Paul^2,3,4, Thoralf Niendorf^1,2,8

¹Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine in the Helmholtz Association, ²Experimental and Clinical Research Center, Max Delbrueck Center for Molecular Medicine and Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, ³NeuroCure Clinical Research Center, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, ⁴Department of Neurology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, ⁵The Swedish National 7T Facility, Lund University Bioimaging Center, Lund University, ⁶Department of Radiography, Medical University of Lublin, ⁷ECOTECH-COMPLEX, Maria Curie-Skłodowska University, ⁸MRI.TOOLS GmbH, ⁹Berlin School of Mind and Brain, Humboldt-Universität zu Berlin

Here, we present a protocol to acquire magnetic resonance (MR) images of multiple sclerosis (MS) patient brains at 7.0 Tesla. The protocol includes preparation of the setup including the radio-frequency coils, standardized interview procedures with MS patients, subject positioning in the MR scanner and MR data acquisition.