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The University of Michigan, Ann Arbor

8 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells

¹Cardiac Surgery, University of Michigan, ²Leon H Charney Division of Cardiology, New York University School of Medicine

In this protocol, derivation of cardiac progenitor cells from both mouse and human embryonic stem cells will be illustrated. A major strategy in this protocol is to enrich cardiac progenitor cells with flow cytometry using fluorescent reporters engineered into the embryonic stem cell lines.

Medicine

Direct Mouse Trauma/Burn Model of Heterotopic Ossification

Jonathan R. Peterson¹, Shailesh Agarwal¹, R. Cameron Brownley¹, Shawn J. Loder¹, Kavitha Ranganathan¹, Paul S. Cederna¹, Yuji Mishina², Stewart C. Wang¹, Benjamin Levi¹

¹Department of Surgery, University of Michigan Medical School, ²Department of Biologic and Materials Sciences, University of Michigan School of Dentistry

An Achilles tenotomy and burn injury model of heterotopic ossification allows for the reliable study of trauma induced ectopic bone formation without the application of exogenous factors.

Medicine

Surgical Treatment for Benign Prostatic Hyperplasia: Holmium Laser Enucleation of the Prostate (HoLEP).

Huan Xu^*1, Xiang Wan^*1, Meng Gu¹, Yanbo Chen¹, Qiling Shi¹, Qi Chen¹, Zhong Wang¹

¹Department of Urology, Shanghai 9th People's Hospital, Shanghai Jiaotong University School of Medicine

Here we present a safe and efficient protocol, holmium laser enucleation of the prostate, to treat benign prostatic hyperplasia.

Cancer Research

A GPC3-targeting Bispecific Antibody, GPC3-S-Fab, with Potent Cytotoxicity

Yanlan Wang^1,2, Jiayu Liu^1,2, Haitao Pan^1,2, Jieyu Xing^1,2, Xiaoqiong Wu^1,2, Qing Li^1,2, Zhong Wang^1,2

¹School of Pharmaceutical Sciences, Sun Yat-Sen University, ²Center for Cellular & Structural Biology, Sun Yat-Sen University

Here, we present a protocol to produce a bispecific antibody GPC3-S-Fab in Escherichia coli. The purified GPC3-S-Fab has potent cytotoxicity against GPC3 positive liver cancer cells.

Chemistry

A New Straightforward Method for Lipophilicity (logP) Measurement using ¹⁹F NMR Spectroscopy

Zhong Wang¹, Benjamin F. Jeffries¹, Hannah R. Felstead¹, Neil J. Wells¹, Elisabetta Chiarparin², Bruno Linclau¹

¹Chemistry, University of Southampton, ²Medicinal Chemistry, Oncology, IMED Biotech Unit, AstraZeneca

A novel and straightforward variation of the shake-flask method was developed for accurate lipophilicity measurement of fluorinated compounds by ¹⁹F NMR spectroscopy.

Bioengineering

Fabrication of the Composite Regenerative Peripheral Nerve Interface (C-RPNI) in the Adult Rat

Shelby R. Svientek¹, Dan C. Ursu¹, Paul S. Cederna^1,2, Stephen W. P. Kemp^1,2

¹Department of Surgery, Division of Plastic Surgery, University of Michigan, Ann Arbor, ²Department of Biomedical Engineering, University of Michigan, Ann Arbor

The following manuscript describes a novel method for developing a biologic, closed loop neural feedback system termed the composite regenerative peripheral nerve interface (C-RPNI). This construct has the ability to integrate with peripheral nerves to amplify efferent motor signals while simultaneously providing afferent sensory feedback.

Medicine

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

Zhaokai Li^*1,2, Liu Liu^*1, Zhong Wang¹

¹Department of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, ²Department of Cardiovascular Medicine, Xiangya Hospital, Central South University

We describe here, the establishment and application of an Tg(Myh6-cre)1Jmk/J /Gt(ROSA)26Sor^{tm38(CAG-GCaMP3)Hze}/J (referred to as αMHC-Cre/Rosa26A-Flox-Stop-Flox-GCaMP3 below) mouse reporter line for cardiac reprogramming assessment. Neonatal cardiac fibroblasts (NCFs) isolated from the mouse strain are converted into induced cardiomyocytes (iCMs), allowing for convenient and efficient evaluation of reprogramming efficiency and functional maturation of iCMs via calcium (Ca²⁺) flux.

Bioengineering

The Muscle Cuff Regenerative Peripheral Nerve Interface for the Amplification of Intact Peripheral Nerve Signals

Shelby R. Svientek¹, Justin P. Wisely¹, Amir Dehdashtian¹, Jarred V. Bratley¹, Paul S. Cederna^1,2, Stephen W. P. Kemp^1,2

¹Department of Surgery, Section of Plastic Surgery, The University of Michigan Health System, ²Department of Biomedical Engineering, The University of Michigan, Ann Arbor

This manuscript provides an innovative method for developing a biologic peripheral nerve interface termed the Muscle Cuff Regenerative Peripheral Nerve Interface (MC-RPNI). This surgical construct can amplify its associated peripheral nerve's motor efferent signals to facilitate accurate detection of motor intent and the potential control of exoskeleton devices.