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Xi’an Jiaotong University

9 ARTICLES PUBLISHED IN JoVE

Neuroscience

Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat

Dongsheng Xu^*1, Jingjing Cui^*1, Jia Wang¹, Zhiyun Zhang¹, Chen She¹, Wanzhu Bai¹

¹Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences

Here, we present a refined protocol to effectively reveal biotinylated dextran amine (BDA) labeling with a fluorescent staining method through a reciprocal neural pathway. It is suitable for analyzing the fine structure of BDA labeling and distinguishing it from other neural elements under a confocal laser scanning microscope.

JoVE Journal

Thermocapillary Convection Space Experiment on the SJ-10 Recoverable Satellite

Li Duan^*1,2, Yongli Yin^*3, Jia Wang^*1, Qi Kang^1,2, Di Wu¹, Huan Jiang¹, Pu Zhang¹, Liang Hu¹

¹National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Sciences, ²School of Engineering Sciences, University of Chinese Academy of Sciences, ³China Astronaut Research and Training Center

A protocol for the space payload design, the space experiment on thermocapillary convection, and analyses of experimental data and images are presented in this paper.

Neuroscience

Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

Jia Wang^*1, Dongsheng Xu^*1, Jingjing Cui¹, Chen She¹, Hui Wang¹, Shuang Wu¹, Ling Zou¹, Jianliang Zhang¹, Wanzhu Bai¹

¹Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences

Here we present a protocol to visualize spatial correlation of calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers and blood vessels in the cranial dura mater using immunofluorescence and fluorescent histochemistry with CGRP and phalloidin, respectively. In addition, the origin of these nerve fibers was retrograde traced with a fluorescent neural tracer.

Chemistry

Optimization of Radiochemical Reactions using Droplet Arrays

Alejandra Rios^1,2, Travis S. Holloway^2,3, Jia Wang^2,4, R. Michael van Dam^1,2,3,4

¹Physics and Biology in Medicine Interdepartmental Graduate Program, University of California Los Angeles (UCLA), ²Crump Institute of Molecular Imaging, UCLA, ³Department of Molecular & Medical Pharmacology, David Geffen School of Medicine, ⁴Department of Bioengineering, UCLA

This method describes the use of a novel high-throughput methodology, based on droplet chemical reactions, for the rapid and economical optimization of radiopharmaceuticals using nanomole amounts of reagents.

Bioengineering

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)

Xingjin Jian^*1,2, Xiaojie Guo^*1,2,3, Jia Wang⁴, Zheng Lin Tan^1,2,6, Xin-hui Xing^1,2,5, Liyan Wang³, Chong Zhang^1,2,5

¹Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, ²Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, ³Luoyang TMAXTREE Biotechnology Co., Ltd., ⁴Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University, ⁵Center for Synthetic & Systems Biology, Tsinghua University, ⁶School of Life Science and Technology, Tokyo Institute of Technology

This protocol describes how to use the Microbial Microdroplet Culture system (MMC) to conduct automated microbial cultivation and adaptive evolution. MMC can cultivate and sub-cultivate microorganisms automatically and continuously and monitor online their growth with relatively high throughput and good parallelization, reducing labor and reagent consumption.

Medicine

Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease

Shuchen Yu¹, Yuchen Zhang¹, Chunhui Zhu², Huan Zhou², Jin Liu², Junyi Sun¹, Ang Li^1,2, Dandan Pei^1,3

¹Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, ²Department of Periodontology, College of Stomatology, Xi’an Jiaotong University, ³Department of Prosthodontics, College of Stomatology, Xi’an Jiaotong University

This article describes two protocols: 1) adjunctive diode laser therapy for treating periodontitis and 2) probiotic Lactobacillus therapy for treating peri-implant disease, with emphasis on the laser usage mode (inside or outside pocket), laser application regimen (single or multiple sessions), and a probiotic protocol of professional and home administration.

Neuroscience

Visualizing the Morphological Characteristics of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle

Jingjing Cui^*1, Shuang Wu^*1, Jia Wang¹, Yuqing Wang¹, Yuxin Su¹, Dongsheng Xu¹, Yihan Liu¹, Junhong Gao¹, Xianghong Jing¹, Wanzhu Bai¹

¹Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences

The protocol shows a method to examine spatial correlation among the pre-synaptic terminals, post-synaptic receptors, and peri-synaptic Schwann cells in the rat medial gastrocnemius muscle using fluorescent immunohistochemistry with different biomarkers, namely, neurofilament 200, vesicular acetylcholine transporter, alpha-bungarotoxin, and S100.

Immunology and Infection

A Periprosthetic Joint Candida albicans Infection Model in Mouse

Chen Yang¹, Jiaxue Zhang¹, Fei Mo², Peipei Zhang¹, Qingqing Li¹, Jiye Zhang¹

¹School of Pharmacy, Health Science Center, Xi’an Jiaotong University, ²Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University

Periprosthetic joint infection (PJI) caused by dangerous pathogens is common in clinical orthopedics. Existing animal models cannot accurately simulate the actual situation of PJI. Here, we established a Candida albicans biofilm-associated PJI mouse model to research and develop new therapeutics for PJI.

Immunology and Infection

Chen Yang¹, Fei Mo², Jiaxue Zhang¹, Peipei Zhang¹, Qingqing Li¹, Jiye Zhang¹

We establish a mouse model of C.albicans-associated catheter-related infection (CRI), in which biofilm forms on the catheter, and the interaction between C.albicans and host correlates well with the clinical CRI. This model helps screen therapies for C.albicans biofilm-associated CRI, laying a foundation for clinical transformation.