Université Paris-Cité, Institut Pasteur, Équipe Labellisée Ligue Contre le Cancer
2 ARTICLES PUBLISHED IN JoVE
Biology
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
Cécile Leduc 1, Audrey Salles 2, Spencer L. Shorte 2, Sandrine Etienne-Manneville 1
1Cell Polarity, Migration and Cancer Unit, UMR 3691, CNRS, Institut Pasteur, 2UTechS Photonic BioImaging (Imagopole) Citech, Institut Pasteur
The overall goal of this methodology is to give the optimal experimental conditions from sample preparation to image acquisition and reconstruction in order to perform 2D dual color dSTORM images of microtubules and intermediate filaments in fixed cells
Biology
Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain
Florent Peglion 1, Franck Coumailleau 1, Sandrine Etienne-Manneville 1
1Cell Polarity, Migration and Cancer Unit, Université Paris-Cité, Institut Pasteur, Équipe Labellisée Ligue Contre le Cancer
We report a technique permitting live imaging of microtubule dynamics in glioblastoma (GBM) cells invading a vertebrate brain tissue. Coupling the orthotopic injection of fluorescently tagged GBM cells into a transparent zebrafish brain with high-resolution intravital imaging allows the measurement of cytoskeleton dynamics during in situ cancer invasion.
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