Université Paris-Cité, Institut Pasteur, Équipe Labellisée Ligue Contre le Cancer

2 ARTICLES PUBLISHED IN JoVE

Biology

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Cécile Leduc¹, Audrey Salles², Spencer L. Shorte², Sandrine Etienne-Manneville¹

¹Cell Polarity, Migration and Cancer Unit, UMR 3691, CNRS, Institut Pasteur, ²UTechS Photonic BioImaging (Imagopole) Citech, Institut Pasteur

The overall goal of this methodology is to give the optimal experimental conditions from sample preparation to image acquisition and reconstruction in order to perform 2D dual color dSTORM images of microtubules and intermediate filaments in fixed cells

Biology

Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain

Florent Peglion¹, Franck Coumailleau¹, Sandrine Etienne-Manneville¹

¹Cell Polarity, Migration and Cancer Unit, Université Paris-Cité, Institut Pasteur, Équipe Labellisée Ligue Contre le Cancer

We report a technique permitting live imaging of microtubule dynamics in glioblastoma (GBM) cells invading a vertebrate brain tissue. Coupling the orthotopic injection of fluorescently tagged GBM cells into a transparent zebrafish brain with high-resolution intravital imaging allows the measurement of cytoskeleton dynamics during in situ cancer invasion.