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National Clinical Research Center for Otolaryngologic Diseases

6 ARTICLES PUBLISHED IN JoVE

Bioengineering

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Yunfeng Chen^*1, Baoyu Liu^*2, Lining Ju^*3, Jinsung Hong^*1, Qinghua Ji^4,5, Wei Chen⁶, Cheng Zhu¹

¹Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, ²Coulter Department of Biomedical Engineering, Georgia Institute of Technology, ³Charles Perkins Centre, The University of Sydney, ⁴Institute of Biophysics, Laboratory of RNA Biology, Chinese Academy of Sciences, ⁵University of Chinese Academy of Sciences, ⁶School of Medicine and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University

We describe a technique for concurrently measuring force-regulated single receptor-ligand binding kinetics and real-time imaging of calcium signaling in a single T lymphocyte.

Bioengineering

Microhoneycomb Monoliths Prepared by the Unidirectional Freeze-drying of Cellulose Nanofiber Based Sols: Method and Extensions

Zheng-Ze Pan^1,2, Hirotomo Nishihara³, Wei Lv¹, Cong Wang^1,2, Yi Luo^1,2, Liubing Dong^1,2, Houfu Song^1,4, Wenjie Zhang², Feiyu Kang^1,2,4, Takashi Kyotani³, Quan-Hong Yang^1,4,5

¹Engineering Laboratory for Functionalized Carbon Materials and Shenzhen Key Laboratory for Graphene-based Materials, Graduate School at Shenzhen, Tsinghua University, ²School of Materials Science and Engineering, Tsinghua University, ³Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, ⁴Tsinghua-Berkeley Shenzhen Institute (TBSI), Tsinghua University, ⁵School of Chemical Engineering and Technology, Tianjin University

Here, we present a general protocol to prepare a variety of microhoneycomb monoliths (MHMs) in which fluid can pass through with an extremely low pressure drop. MHMs obtained are expected to be used as filters, catalyst supports, flow-type electrodes, sensors and scaffolds for biomaterials.

Neuroscience

Intrathecal Delivery of Antisense Oligonucleotides in the Rat Central Nervous System

Yi Chen^*1, Curt Mazur^*2, Yi Luo^*1, Linhong Sun¹, Mingdi Zhang¹, Alex McCampbell¹, Giulio Srubek Tomassy¹

¹Biogen, Inc., ²Ionis Pharmaceuticals

Here, we describe a method for delivering drugs to the rat central nervous system by implanting a catheter into the lumbar intrathecal space of the spine. We focus on the delivery of antisense oligonucleotides, though this method is suitable for delivery of other therapeutic modalities as well.

Medicine

Indocyanine Green-Guided Intraoperative Imaging to Facilitate Video-Assisted Retroperitoneal Debridement for Treating Acute Necrotizing Pancreatitis

Li Huang¹, Wei Chen¹, Jiancong Chen¹, Dong Chen¹, Kunsong Zhang¹, Jianpeng Cai¹, Hong Peng¹, Chensong Huang¹, Guangyan Zeng¹, Mingjian Ma¹, Jiahua Liang¹, Borui Xu¹, Xiaoyu Yin¹, Jiaming Lai¹, Lijian Liang¹

¹Department of Pancreatobiliary Surgery, The First Affiliated Hospital, Sun Yat-sen University

This protocol presents Indocyanine Green-guided video-assisted retroperitoneal debridement (ICG-guided VARD) for treating severe acute necrotizing pancreatitis.

Medicine

The Miniature Pig: A Large Animal Model for Cochlear Implant Research

Xiaojun Ji^*1,2,3,4, Yi Luo^*1,2,3,4, Weiwei Guo^1,2,3,4, Fei Ji^1,2,3,4, Shuolong Yuan^1,2,3,4, Liangwei Xu^1,2,3,4, Wei Chen^*1,2,3,4

¹College of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, ²National Clinical Research Center for Otolaryngologic Diseases, ³Key Lab of Hearing Science, Ministry of Education, ⁴Beijing Key Lab of Hearing Impairment for Prevention and Treatment

Miniature pigs (mini-pigs) are an ideal large animal model for research into cochlear implants. Cochlear implantation surgery in mini-pigs can be utilized to provide initial evidence of the safety and potential performance of novel electrode arrays and surgical approaches in a living system similar to human beings.

Medicine

Endoscopic Balloon Dilatation of the Eustachian Tube via the Soft Palate Approach in Miniature Pigs

Hui Song^*1,2,3,4, Jianping Jia^*1,2,3,4, Danheng Zhao^*1,2,3,4, Mengyuan Guo^1,2,3,4, Fangyuan Wang^1,2,3,4, Kun Hou^1,2,3,4, Nan Jiang⁵, Zhenhao Fu^1,2,3,4, Jingcheng Zhou^1,2,3,4, Haoze Zhang^1,2,3,4, Yulin Ding^1,2,3,4, Yuting Yang⁵, Chen Chen⁵, Xinyuan Jia^1,2,3,4, Zhaohui Hou^1,2,3,4, Shiming Yang^1,2,3,4

¹Senior Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Chinese PLA Medical School, ²National Clinical Research Center for Otolaryngologic Diseases, ³Key Laboratory of Hearing Science, Ministry of Education, ⁴Beijing Key Laboratory of Hearing Impairment for Prevention and Treatment, ⁵Liaoning University of Traditional Chinese Medicine

In this study, we present a protocol to explore an eustachian tube surgery approach via the soft palate in miniature pigs. The surgical procedure is simple, with short surgical time, and wound healing is rapid; it is thus a good choice for procedures such as balloon dilation of the eustachian tube.