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Chinese Academy of Agricultural Sciences, Ministry of Agriculture

5 ARTICLES PUBLISHED IN JoVE

Developmental Biology

A Mini-Invasive Internal Fixation Technique for Studying Immobilization-Induced Knee Flexion Contracture in Rats

Shihai Jiang^*1, Xiaoyou Yi^*3, Yuansen Luo², Dongjie Yu¹, Yuangao Liu¹, Fei Zhang^1,4, Lei Zhu², Kun Wang¹

¹Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, ²Department of Plastic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, ³Department of Bone Surgery, Tungwah Hospital of Sun Yat-sen University, ⁴Department of Othopaedic Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University

Here, we present a protocol to describe a minimally invasive technique for knee joint immobilization in a rat model. This reproducible protocol, basing on muscle-gap separation modus and the mini-incision skill, is suitable for studying the underlying molecular mechanism of acquired joint contracture.

Medicine

Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis

Kaiyue Zhang¹, Chen Wang¹, Ran Wang², Shang Chen¹, Zongjin Li¹

¹Nankai University School of Medicine, ²State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University

This protocol describes the establishment of a tumor-bearing mouse model to monitor tumor progression and angiogenesis in real-time by dual bioluminescence imaging.

Medicine

Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes

Yun Liu^*1, Yin Liang^*1, Mengxue Wang^*1, Chen Wang¹, Heng Wei², Keiji Naruse¹, Ken Takahashi¹

¹Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, ²Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

We present a model of ischemic heart disease using cardiomyocytes derived from human induced pluripotent stem cells, together with a method for quantitative evaluation of tissue damage caused by ischemia. This model can provide a useful platform for drug screening and further research on ischemic heart disease.

Immunology and Infection

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

Marieke de Korte^1,2, Armand Keating^1,2,4, Chen Wang^1,3

¹Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, ²Krembil Research Institute, Toronto Western Hospital, University Health Network, ³Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital, Sinai Health Systems, ⁴Medical Oncology and Hematology, Princess Margaret Cancer Centre

This is a step-by-step guide for using a commercially available rotary cell culture system to culture lymphocytes in simulated microgravity using specialized disposable culture vessels. This culturing method may be applied to any suspension-type cell culture.

Environment

Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples

Weiting Jiao^1,2, Tingting Shen², Luyao Wang¹, Lei Zhu^1,3, Qing X. Li⁴, Chen Wang³, Hongping Chen³, Rimao Hua¹, Xiangwei Wu¹

¹Key Laboratory of Agri-food Safety of Anhui Province, School of Resource & Environment, Anhui Agricultural University, ²State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Agricultural University, ³Key Laboratory of Tea Quality and Safety & Risk Assessment, Tea Research Institute, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, ⁴Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa

The present protocol describes the contamination of pyrrolizidine alkaloids (PAs) in tea samples from PA-producing weeds in tea gardens.