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Research Laboratory for Pediatric Surgery

1 ARTICLES PUBLISHED IN JoVE

Medicine

Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates

Hans Christian Schmidt^1,2,3, Johanna Hagens^1,2,3, Pauline Schuppert^1,2, Clara Philippi^1,2, Konrad Reinshagen^1,2, Christian Tomuschat^1,2

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, ²Research Laboratory for Pediatric Surgery, ³Research Animal Facility, University Medical Center Hamburg-Eppendorf

For the observation of murine neonatal bile duct disorders, an intact bile duct and efficient preparation are required. Therefore, a new approach for isolating the entire extrahepatic bile duct system in murine neonates was successfully developed while maintaining the integrity of the bile duct.