Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse NeonatesHans Christian Schmidt 1,2,3, Johanna Hagens 1,2,3, Pauline Schuppert 1,2, Clara Philippi 1,2, Konrad Reinshagen 1,2, Christian Tomuschat 1,2
1Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, 2Research Laboratory for Pediatric Surgery, 3Research Animal Facility, University Medical Center Hamburg-Eppendorf
For the observation of murine neonatal bile duct disorders, an intact bile duct and efficient preparation are required. Therefore, a new approach for isolating the entire extrahepatic bile duct system in murine neonates was successfully developed while maintaining the integrity of the bile duct.