JoVE Logo
Centro de recursos académicos

Iniciar sesión

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

DOI :

10.3791/1656-v

December 31st, 2009

December 31st, 2009

12,245 Views

1Department of Mechanical Engineering, University of Louisville, 2Department of Bioengineering, University of Louisville

An automated microfluidic device was developed for circulating nucleated cell enrichment from peripheral blood via erythrocyte lysis that ensures isolation of high quality sample without cell loss.

Tags

Automated Microfluidic Blood Lysis

-- Vistas

Videos relacionados

article

Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies

article

Microfluidic Applications for Disposable Diagnostics

article

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions

article

Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice

article

Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood

article

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

article

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

article

A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry

article

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

article

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

JoVE Logo

Privacidad

Condiciones de uso

Políticas

Investigación

Educación

ACERCA DE JoVE

Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados