establishment of an ulcerative coloproctitis-colorectal cancer (uc-crc) model to evaluate the therapeutic effect of chinese medicine

Decocción de Liujunzi: Un tratamiento eficaz para la prevención del CCU-CRC

Colorectal cancer (CRC) is a common gastrointestinal malignancy, the third most common malignancy, and the second most common cause of death in the world, accounting for 10% of the global cancer incidence and 9.4% of the total cancer-related death1,2. Genetic factors, chronic inflammation, high-fat diet, diabetes, and abnormal intestinal flora are risk factors for CRC3,4. Among them, inflammatory bowel disease, especially ulcerative coloproctitis (UC), is a clear risk factor for CRC5,6. UC-associated CRC (UC-CRC) is a transition process of inflammation, atypical hyperplasia, and cancer based on chronic inflammation of the colorectum, which is different from the typical adenoma-adenocarcinoma development model of CRC7,8. Compared with the general population, the risk of CRC is approximately 10-40 times higher in patients with inflammatory bowel disease9.
Currently, surgery is still the standard treatment for CRC, and depending on the location and stage of the tumor, radiation therapy, systemic drug therapy, or a combination of both are possible10. Although these traditional treatment modalities have made great progress, due to the high heterogeneity and recurrence rate of CRC, the prognosis is poor, and the treatment effect is not ideal11,12. Therefore, early detection, early diagnosis, and comprehensive treatment are key to improving the survival rate of CRC patients, and it is particularly important to pay attention to the transformation of UC to CRC. Over the years, traditional Chinese medicine (TCM) has attracted much attention in the treatment of UC-CRC or chronic gastritis due to its limited side effects and significant efficacy. Based on dialectical treatment, famous Chinese medicine practitioners of various generations have created a large number of classic prescriptions, such as Huangqi Jianzhong decoction13, Sijunzi decoction14, and Sishen pill15.
Liujunzi decoction (LJZD) originated from the works of Yi Xue Zheng Zhuan compiled in the Ming Dynasty and is a classic prescription in TCM16. As shown in Table 1, LJZD consists of six traditional herbs, including Codonopsis pilosula (Franch.) Nannf. (Dangshen), Poria cocos (Schw.) Wolf (Fuling), Atractylodes macrocephala Koidz. (Baizhu), Glycyrrhiza uralensis Fisch. (Gancao), Citrus reticulata Blanco (Chenpi) and Pinellia ternata (Thunb.) Breit (Banxia), which has the effect of replenishing qi and strengthening the spleen, drying dampness, and resolving phlegm. In modern clinical practice, it is often used to treat chronic gastritis, gastric ulcers, and duodenal ulcers. Modern pharmacological research has shown that LJZD and modified LJZD have high application value in the adjuvant treatment of UC and digestive tract cancer17,18,19.
At present, there are many ways to construct UC-CRC mouse models, but the azoxymethane (AOM)/dextran sulfate sodium (DSS) induced mouse model is the most widely used UC-CRC model; the clinical symptoms, morphological, and pathological observations have proved that the model is very similar to human UC-CRC20,21. The basic principle is to first induce carcinogenesis with chemical carcinogen AOM and then continuously expose mice to the inflammatory stimulation environment of DSS to simulate the continuous damage and repair of intestinal mucosal epithelium, thereby constructing a UC-CRC mouse model22. The aim of this study is to establish a mouse model of UC-CRC by intraperitoneal injection of AOM and cyclic stimulation of DSS in the short term and to evaluate the effect of the drug and the molecular mechanism of LJZD on UC-CRC in order to provide a scientific basis for the treatment of UC-CRC.

Autor destacado: La decocción de Liujunzi como tratamiento tradicional chino para el cáncer de coloproctitis

Establecimiento de un modelo de cáncer de coloproctitis en ratones y evaluación del efecto terapéutico de la medicina china

Watch this Scientific Journal Video about Establishment of an Ulcerative Coloproctitis-Colorectal Cancer UC-CRC Model to Evaluate the Therapeutic Effect of Chinese Medicine at JoVE.com

Establecimiento de un modelo de coloproctitis ulcerosa-cáncer colorrectal (CCR-CU) para evaluar el efecto terapéutico de la medicina china

Para empezar, coloca suavemente el ratón con el vientre hacia arriba y la cabeza ligeramente hacia abajo. Agarre la piel de la espalda para asegurar el área abdominal e inserte una aguja de jeringa de un mililitro, un centímetro a la derecha de la línea media del abdomen en la intersección de la línea, conectando ambos muslos. Inserte gradualmente la aguja de la jeringa de un mililitro a una profundidad de tres a cinco milímetros debajo de la piel.Inserte la aguja aproximadamente de 0,3 a 0,5 milímetros en la cavidad abdominal en un ángulo de 45 grados. Una vez que la punta de la aguja pasa a través del músculo abdominal, observe una disminución repentina de la resistencia. Inyecte lentamente 0,1 mililitros de la solución inyectable de azoximetano por cada 10 gramos de peso corporal en los ratones.Posteriormente, retraiga la jeringa suavemente. Luego agregue 500 mililitros de agua destilada estéril a 10 gramos de DSS. Para preparar una solución 2%DSS.Mezcle la solución con un mezclador de vórtice y guárdela a cuatro grados centígrados hasta su uso, administre la solución preparada de LJZD y ácido cinco aminosalicílicos diariamente a un tiempo constante a través de sonda gástrica a los grupos respectivos en la semana siete y la semana 15.

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Research

JoVE Journal

Medicine

Establishment of an Ulcerative Coloproctitis-Colorectal Cancer (UC-CRC) Model to Evaluate the Therapeutic Effect of Chinese Medicine

44 vistas. Para empezar, coloca suavemente el ratón con el vientre hacia arriba y la cabeza ligeramente hacia abajo. Agarre la piel de la espalda para asegurar el área abdominal e inserte una aguja de jeringa de un mililitro, un centímetro a la derecha de la línea media del abdomen en la intersección de la línea, conectando ambos muslos. Inserte gradualmente la aguja de la jeringa de un mililitro a una profundidad de tres a cinco milímetros debajo de la piel.Inserte la aguja aproximadament...

Video: Establecimiento de un modelo de coloproctitis ulcerosa-cáncer colorrectal (CCR-CU) para evaluar el efecto terapéutico de la medicina china

Establishment of Coloproctitis Cancer Model in Mice and Evaluation of Therapeutic Effect of Chinese Medicine

Colorectal cancer (CRC) is a common malignancy of the digestive system and has become the third most common malignancy worldwide and the second leading cause of malignancy-related death. Ulcerative coloproctitis (UC) is a precancerous lesion, and UC-associated CRC (UC-CRC) is the most common subtype of CRC. Therefore, a reasonable UC-CRC model is the cornerstone and guarantee of new drug development. Traditional Chinese medicine (TCM) has been widely used in the treatment of UC-CRC due to its good efficacy. As a classic tonic prescription of TCM, Liujunzi decoction (LJZD) has been widely used in the treatment of UC-CRC. In this study, a UC-CRC model was established by combining azomethane and dextran sulfate sodium, and the LJZD was administered. The data confirmed that LJZD can effectively inhibit cancer transition in UC-CRC by using mouse body weight, colorectal length, pathological and inflammatory factors, colorectal barrier function, and cancer markers. This protocol provides a system for evaluating the efficacy of TCM in the prevention and treatment of UC-CRC.

This protocol provides a mouse model of ulcerative coloproctitis-associated colorectal cancer induced by azomethane combined with dextran sulfate sodium. The model was used to evaluate the efficacy of traditional Chinese medicine compounds in the prevention and treatment of colorectal cancer.

Colorectal cancer (CRC) is a common malignancy of the digestive system and has become the third most common malignancy worldwide and the second leading ...