August 16th, 2014
•This protocol combines electrospinning and microspheres to develop tissue engineered scaffolds to direct neurons. Nerve growth factor was encapsulated within PLGA microspheres and electrospun into Hyaluronic Acid (HA) fibrous scaffolds. The protein bioactivity was tested by seeding the scaffolds with primary chick Dorsal Root Ganglia and culturing for 4-6 days.
Tags
Videos relacionados
Electrospinning Fundamentals: Optimizing Solution and Apparatus Parameters
Elastomeric PGS Scaffolds in Arterial Tissue Engineering
Axon Stretch Growth: The Mechanotransduction of Neuronal Growth
Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells
Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy
Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation
Melt Electrospinning Writing of Three-dimensional Poly(ε-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications
Fabrication of Decellularized Cartilage-derived Matrix Scaffolds
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados