November 8th, 2014
•Primary cell culture using intact tissue organoids provides a model system that mimics the multi-cellular in vivo microenvironment. We developed a serum-free primary breast epithelium tissue culture model that perpetuates mixed cell culture lineages and exhibits differentiated morphology, without enzymatic tissue disruption. Breast organoids remain viable for >6 months.
Videos relacionados
Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation
Generation of Single-Cell Suspensions from Mouse Neural Tissue
Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography
Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster
Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood
A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados