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Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

13.6K Views

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07:08 min

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December 23rd, 2015

DOI :

10.3791/53445-v

December 23rd, 2015

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13,625 Views

Daniel L. Kober*¹^,²^,³, Zeynep Yurtsever*²^,³^,⁴^,⁵, Thomas J. Brett²^,³^,⁵^,⁶^,⁷

¹Molecular Microbiology and Microbial Pathogenesis Program, Washington University School of Medicine, ²Department of Internal Medicine, Washington University School of Medicine, ³Drug Discovery Program in Pulmonary and Critical Care Medicine, Washington University School of Medicine, ⁴Biochemistry Program, Washington University School of Medicine, ⁵Center for the Investigation of Membrane Excitability Diseases, Washington University School of Medicine, ⁶Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, ⁷Department of Cell Biology and Physiology, Washington University School of Medicine

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Mammalian Cell Expression

Extracellular Glycoproteins

Crystallization

Transient Transfection

Nickel Affinity Chromatography

Protein Production

Protein Yield

Glycan Maturation

X ray Crystallography

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