A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

0:56

Assay Mixture Preparation

2:24

Droplet Generation and PCR Plate Setup

5:17

Thermal Cycling and Droplet Reading

8:01

Data Analysis and Reporting

9:46

Results: Duplex Digital PCR is a Robust Assay for Simultaneous Quantification of General and Human-associated Fecal Contamination

11:18

Conclusion