Transfection and Immunofluorescent Staining for Endogenous Centromere Kinetochore Protein Detection
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Results: Representative Immunoflurescence Analysis of Centromere Protein-A (CENP-A) Protein Expression
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Conclusion
Transcripción
The overall goal of this indirect immunoflorescent assay is to optimize the localization and quantification of endogenous and exogenous centromere-kinetochore proteins, including centromere protein A and flag-tagged exogenous CENP-A proteins in hu
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Here we report protocols to detect endogenous and exogenous centromere-kinetochore proteins in human cells and quantify these protein levels at centromeres-kinetochores by indirect immunofluorescent staining through the use of fixation (paraformaldehyde, acetone, or methanol fixation).