Name: using adhesive patterning to construct 3d paper microfluidic devices
Uploaded: 2016-04-01T14:00:00
Description: Watch this Scientific Journal Video about Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices at JoVE.com

This work is supported by a fund from Bourns College of Engineering of University of California, Riverside. BK received a scholarship from the Lung-Wen Tsai Memorial Award in Mechanical Design.

1. Planar 4-layer Device (Stacked Layers) Construction
<ol>
	<li>Print arrays of each layer of the device9 onto each piece of filter paper using a solid ink printer.11,12 Place each filter paper on a hotplate at 170 &#176;C for 2 min. This will melt the wax-based ink and allow it to fully penetrate the thickness of the paper, forming hydrophobic barriers. 
	NOTE: The exact designs used are available as supplemental files.</li>
	<li>Remove filter paper from hotplate and allow it to cool to RT....

<ol>
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A., Kauffman, P., Lutz, B., Yager, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transport+in+two-dimensional+paper+networks.">Transport in two-dimensional paper networks.</a> Microfluid Nanofluidics. 10, 29-35 (2011).</li><li>Govindarajan, A. V., Ramachandran, S., Vigil, G. D., Yager, P., Bohringer, K. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+low+cost+point-of-care+viscous+sample+preparation+device+for+molecular+diagnosis+in+the+developing+world;+an+example+of+microfluidic+origami.">A low cost point-of-care viscous sample preparation device for molecular diagnosis in the developing world; an example of microfluidic origami.</a> Lab Chip. 12, 174-181 (2012).</li><li>Schilling, K. M., Jauregui, D., Martinez, A. 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The above protocols use perforated metal sheets as stencils for applying aerosol adhesives to construct planar and nonplanar 3D paper microfluidic devices. In planar devices, this has the advantage of allowing devices to be completely unfolded after the adhesive has dried without destroying the device. In other adhesive based construction techniques, this is almost impossible, although, some designs allow for partial destructive disassembly by unpeeling two halves held together with a removable adhesive.14 Adh...

In recent years, paper microfluidics has garnered considerable popularity for its potential to provide low-cost point of care (POC) diagnostic devices.1-3 POC devices offer functionality similar to those of lab-based tests in a format that allows results to be obtained relatively quickly. POC devices made from paper are low-cost, lightweight, and easy-to-use alternatives to expensive microfluidic chips and miniaturized laboratories, making them ideal for use in resource-limited settings. The most common paper microfluidic devices are one-dimensional lateral flow devices, but planar three-dimensional (3D) paper microfluidic devices hold promise to provide multiplexed diagnostic devices4 that take up a much smaller footprint than would be required by a 2D device5 and correspondingly use a smaller sample volume.
Initially, planar 3D paper microfluidic devices were assembled individually, layer-by-layer with patterned paper layers alternating with laser-cut double-sided tape. Carefully aligned holes cut in the tape layer were filled with cellulose powder to ensure inter-layer fluid transport.4 A number of alternate methods were subsequently developed,6-9 each improving different aspects of the devices. In particular, by eschewing adhesives, devices could be folded via origami techniques with layers held together by an external clamp.8 This eliminates any potential adhesive interference in a diagnostic test and allows the device to be unfolded post-use, potentially allowing even smaller sample volumes by displaying results internally. Alternatively, by using an aerosol adhesive applied between each paper layer, sheets of devices could be assembled simultaneously, without time-consuming patterning and alignment of tape.9
However, by applying an aerosol adhesive through a stencil, it is possible to gain the benefit of both of these techniques. By spraying the adhesive through a stencil, only a fraction of the adhesive is applied to the device, minimizing any potential interference with interlayer fluid transfer. Additionally, with careful stencil selection, a pattern of adhesive can be applied that results in semi-permanent adhesive bonding, allowing devices to be unfolded after use, while still providing sufficient interlayer contact to allow fluid to wick between layers.
Finally, applying aerosol adhesives through a stencil eases the construction of nonplanar 3D paper microfluidic devices, by minimizing the amount of adhesive applied to adjacent faces that may require frequent folding and unfolding during construction.10 Additionally, the use of patterned adhesive enables device to be unfolded after use for more convenient storage. Nonplanar 3D paper microfluidic devices are expected to be used for tasks that would otherwise be impossible in a planar 3D device. Figure 1 depicts the general process flow used to construct both planar and nonplanar 3D devices.

<table border="0" cellpadding="0" cellspacing="0" width="585">
	<tbody>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Camera</td>
			<td style="width:143px;">Nikon</td>
			<td style="width:119px;">D5100</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Solid-ink printer</td>
			<td style="width:143px;">Xerox</td>
			<td style="width:119px;">ColorQube 8880</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Hotplate</td>
			<td style="width:143px;">Torrey Pines</td>
			<td style="width:119px;">HS60</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Humidity chamber</td>
			<td style="width:143px;">Electro-Tech Systems</td>
			<td style="width:119px;">5503-E</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Spray adhesive</td>
			<td style="width:143px;">3M</td>
			<td style="width:119px;">62497749309</td>
			<td style="width:160px;">Super 77 (16.75 oz can)</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Filter paper</td>
			<td style="width:143px;">Whatman</td>
			<td style="width:119px;">Grade 4</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Perforated steel sheet</td>
			<td style="width:143px;">MetalsDepot</td>
			<td style="width:119px;">PS16116</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Tartrazine</td>
			<td style="width:143px;">Sigma-Aldritch</td>
			<td style="width:119px;">T0388</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Allura Red</td>
			<td style="width:143px;">Sigma-Aldritch</td>
			<td style="width:119px;">458848</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Erioglaucine disodium salt</td>
			<td style="width:143px;">Sigma-Aldritch</td>
			<td style="width:119px;">861146</td>
			<td style="width:160px;"></td>
		</tr>
	</tbody>
</table>

using adhesive patterning to construct 3d paper microfluidic devices

We demonstrate the use of patterned aerosol adhesives to construct both planar and nonplanar 3D paper microfluidic devices. By spraying an aerosol adhesive through a metal stencil, the overall amount of adhesive used in assembling paper microfluidic devices can be significantly reduced. We show on a simple 4-layer planar paper microfluidic device that the optimal adhesive application technique and device construction style depends heavily on desired performance characteristics. By moderately increasing the overall area of a device, it is possible to dramatically decrease the wicking time and increase device success rates while also reducing the amount of adhesive required to keep the device together. Such adhesive application also causes the adhesive to form semi-permanent bonds instead of permanent bonds between paper layers, enabling single-use devices to be non-destructively disassembled after use. Nonplanar 3D origami devices also benefit from the semi-permanent bonds during folding, as it reduces the likelihood that unrelated faces may accidently stick together. Like planar devices, nonplanar structures see reduced wicking times with patterned adhesive application vs uniformly applied adhesive.

The 4-layer device tests were performed in a sealed chamber, shielding them from any wind or breezes that might cause excessive evaporation of the limited deposited fluid volume. The majority of the wicking in the 4-layer devices is in the middle layers of the device, so differences in wicking speeds due to evaporation were expected to be minimal. Additionally, there is minimal lateral wicking, with only 13 mm between the inlet and any individual outlet, suggesting that variations in wick...

Watch this Scientific Journal Video about Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices at JoVE.com

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices

We demonstrate the use of patterned aerosol adhesives to construct 3D paper microfluidic devices. This method of adhesive application forms semi-permanent bonds between layers, enabling single-use devices to be non-destructively disassembled after use and to ease folding complex nonplanar structures.

We demonstrate the use of patterned aerosol adhesives to construct both planar and nonplanar 3D paper microfluidic devices. By spraying an aerosol ...

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