Results: Absorption and Fluorescence Spectroscopy Changes upon DNA Hybridization
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Conclusion
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The overall goal of this experiment is to visualize the energy transfer from one dye modified DNA strand to another. This method can help on the key questions in the field of molecular biology. Such as RNA interference by monitoring siRNA processi
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Photostable cyanine dyes are attached to oligonucleotides to monitor hybridization by energy transfer.