Evaluation of Cell Migration with Scratch-induced Directional Wounding Assay
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Results: Western Blot Analysis and Scratch-induced Directional Wounding Assay to Evaluate Cell Migration
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Conclusion
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The overall goal of this RNA Transfection assay is to use HSP27 specific small interfering RNA to assess the potential role of HSP27 in corneal epithelial wound healing. This method can help answer key questions regarding the the function of Heat
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Herein, we present a protocol to use heat shock protein 27 (HSP27)-specific small interfering RNA to assess the function of HSP27 during corneal epithelial wound healing. RNA interference is the best method for effectively knocking-down gene expression to investigate protein function in various cell types.