Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

0:51

Construction of the PUF RNA-binding Domain Scaffold with Customized RNA Binding Specificity by Overlapping PCR

2:05

Transfection of Engineered Splicing Factors (ESFs) for Modulation of Exon Inclusion and Alternative Splice-site Use

3:43

Cell Harvest and Identification of Spliced Isoforms

6:24

Modulation of Endogenous Bcl-x Splicing and Apoptosis by ESFs

8:23

Results: Subcellular Localization of ESFs in Transfected HeLa Cells

9:30

Conclusion