Collection and Fixation of Developing and Fully Formed Plant Structures
2:04
Dissection and Dehydration of Developing and Fully Formed Plant Structures
3:02
Mounting and Preparing Plant Tissues for SEM Observation of Developing and Fully Formed Plant Structures
4:20
Study of Cyst Behavior of Saprolegnia (Oomycetes) on Different Surfaces
6:04
Study of Herbarium Fungal Spores of Phellorinia herculanea under SEM
7:20
Results: Recovery of Plant Development, Differential Spine Growth of Saprolegnia parasitica, and Nurse Cells in Spores of Phellorinia herculanea f. stellata
9:01
Conclusion
Transcribir
The goal of this methodology is to handle delicate tissues from plants, oomycetes, and fungi, for their optimal observation under a Scanning Electron Microscope, or SEM. This method can help us answering questions regarding practically any fungi,
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Problems in the processing of biological samples for scanning electron microscopy observation include cell collapse, treatment of samples from wet microenvironments and cell destruction. Low-cost and relatively rapid protocols suited for preparing challenging samples such as floral meristems, oomycete cysts, and fungi (Agaricales) are compiled and detailed here.