Isolation of Peripheral Blood Mononuclear Cells (PB MNCs)
3:03
Expansion of PB MNCs in Erythroid Medium
4:07
PB MNCs Nucleofection and Reprogramming
8:00
Results: Generation of Integration-free iPSCs from PB MNCs
8:48
Conclusion
Transcripción
The overall goal of this procedure is to generate iPS cells from adult human peripheral blood mononuclear cells using non-integrating episomal vectors. This protocol can help scientists in the stem cell field who wish to generate iPS cells for dis
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This protocol describes a detailed method for efficient generation of integration-free iPSCs from human adult peripheral blood cells. With the use of four oriP/EBNA-based episomal vectors to express the reprogramming factors, KLF4, MYC, BCL-XL, or OCT4 and SOX2, thousands of iPSC colonies can be obtained from 1 mL of peripheral blood.