Construction and Expression of Serially Truncated Recombinant Proteins
3:45
Dot-blot Hybridization
6:24
Results: Recognition of the Epitope Region by Dot-blot Hybridization
7:44
Conclusion
Transcribir
The overall goal of this protocol is to identify a monoclonal antibody-recognized linear B-cell epitope using serially truncated recombinant proteins and peptide scanning by dot-blot hybridization. This method can help answer key questions concern
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Here, the authors present a simple and efficient protocol to define a linear antigenic epitope using a purified monoclonal antibody and peptide scanning through dot-blot hybridization. The identified epitope can then be used in therapeutic and diagnostic applications.