August 22nd, 2019
•The BSelex method to identify and recover individual antigen-specific antibodies from human peripheral blood mononuclear cells combines flow cytometry with single cell PCR and cloning.
Tags
Videos relacionados
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions
Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados