Removal of Patch from the Needle Array and Patch Maturation
3:35
Results: 3D Bioprinted Spheroids Exhibit Mechanical Integration
3:55
Conclusion
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The purpose of this video is to demonstrate 3D bioprinting of cardiac tissue without the use of biomaterials, using only cells. Firstly, cardiomyocytes, endothelial cells and fibroblasts are isolated, counted and mixed at desired cell ratios. They
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This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease.