Coating of Bacteria with NHS-biotin/Streptavidin-conjugated Magnetic Beads
4:38
Bone Marrow-derived Macrophages (BMDM) Infection
6:18
Isolation of S. typhimurium-containing Phagosomes
8:21
Results: Successful Biotinylation of Bacterial and Isolation of Bacterial-containing Phagosomes
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Conclusion
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The overall goal of this procedure is to isolate intact and enriched bacteria containing Phagosomes. This method can help us answer key questions in the field of Immunology such as changes in Phagosome maturation and antigen processing when pathog
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We describe here a simple and quick method for the isolation of Salmonella typhimurium-containing phagosomes from macrophages by coating the bacteria with biotin and streptavidin.