Metaphase I (MI) or Metaphase II (MII) Oocyte Collection
5:17
Oocyte Denuding and Zona Pellucida Removal
7:05
MI and MII Oocyte Chromatin Spreads
8:08
Results: Crossover Formation in C57B/6J Oocytes
9:52
Conclusion
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The overall goal of this chromatin spread preparation is to chronologically visualize the dynamic localization patterns of chromatin-bound proteins and chromosome morphology of oocytes throughout mammalian oogenesis. This method can help answer ke
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Oogenesis in mammals is known to be error-prone, particularly due to chromosome missegregation. This manuscript describes chromatin spread preparation methods for mouse prophase, metaphase I and II-staged oocytes. These fundamental techniques allow for the study of chromatin-bound proteins and chromosome morphology throughout mammalian oogenesis.