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Genetics

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Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

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10:39 min

February 26th, 2018

February 26th, 2018

14,983 Views

0:04

Title

0:59

Harvesting Fetal or Neonatal Ovaries

3:55

Metaphase I (MI) or Metaphase II (MII) Oocyte Collection

5:17

Oocyte Denuding and Zona Pellucida Removal

7:05

MI and MII Oocyte Chromatin Spreads

8:08

Results: Crossover Formation in C57B/6J Oocytes

9:52

Conclusion

Transcribir

The overall goal of this chromatin spread preparation is to chronologically visualize the dynamic localization patterns of chromatin-bound proteins and chromosome morphology of oocytes throughout mammalian oogenesis. This method can help answer ke

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Oogenesis in mammals is known to be error-prone, particularly due to chromosome missegregation. This manuscript describes chromatin spread preparation methods for mouse prophase, metaphase I and II-staged oocytes. These fundamental techniques allow for the study of chromatin-bound proteins and chromosome morphology throughout mammalian oogenesis.

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