An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector
Cytofluorimetric Analysis of Transduced Primary Keratinocytes
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Quantitative RT-PCR (qRT-PCR)
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Results: Sleeping Beauty System Achieves Stable Integration of a GOI in the Human Genome
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Conclusion
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The overall goal of this method is to achieve stable integration of a gene of interest into the human genome of primary cells by the transcriptionally regulated Sleeping Beauty system packaged into integration defective lentiviral vectors. This me
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This protocol describes a method to achieve stable integration of a gene of interest into the human genome by the transcriptionally regulated Sleeping Beauty system. Preparation of the integration of defective lentiviral vectors, the in vitro transduction of human cells, and the molecular assay on transduced cells are reported.