Isolation of Tumor-secreted Extracellular Vesicles (EVs)
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Generation of an Orthotopic Xenograft Mouse Model of Osteosarcoma
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Assessment of Lung Nodule Number
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Histological Analysis of GFP-positive MSCs in Mouse Tibias
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Results: Analysis of Mice Injected with EV-educated MSCs
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Conclusion
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The overall goal of this experimental procedure is to develop a preclinical mouse model to study the role of extracellular vesicle-mediated communication between tumor and mesenchymal stem cells. This method can help answer several key questions i
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Direct injection of cancer-derived extracellular vesicles (EVs) leads to reprogramming of bone marrow supporting tumor progression; however, which cells mediate this effect is unclear. Herein, we describe a step-by-step protocol to investigate EV-mediated tumor-mesenchymal stem cell (MSC) interactions in vivo, revealing a crucial role for EV-educated MSCs in metastasis.