Preparation of Optical Setup and Calibration of Microencapsulated SNARF-1
8:19
Preparation for Injection
8:59
Injection
10:41
In Vivo Visualization
11:55
Results: In Vivo Measurements of Zebrafish Plasma pH under Hypercapnia
12:21
Conclusion
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The overall goal of this procedure is to demonstrate a technique for introduction of microparticles into the circulatory system of adult zebrafish by injection into the fish kidney. The introduced microparticles are further visualized in the vascu
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This article demonstrates the principles of a quick, minimally invasive injection of fluorescent microparticles into the circulatory system of small fishes and the in vivo visualization of the microparticles in fish blood.