September 7th, 2018
•In this paper, we describe a protocol to characterize T-dependent and T-independent immunoglobulin (Ig) isotype responses in mice using ELISA. This method used alone or in combination with flow cytometry will allow researchers to identify differences in B cell-mediated Ig isotype responses in mice following T-dependent and T-independent antigen immunization.
Tags
Videos relacionados
Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae
Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice
Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados