October 16th, 2018
•Here, we present a protocol for the production and pre-clinical testing of murine CD19 CAR T cells by retroviral transduction and utilization as a therapy against established syngeneic A20 B-cell lymphoma in BALB/c mice with or without lymphodepleting pre-conditioning.
Videos relacionados
A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical Therapies
Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
A Syngeneic Pancreatic Cancer Mouse Model to Study the Effects of Irreversible Electroporation
Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression
A Spheroid Killing Assay by CAR T Cells
Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy
Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma
A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados