Verification of the Direct Target Gene of a miRNA of Interest
6:37
Luciferase Assay
7:52
Results: miRNA-107 Negatively Regulates Proliferation of PANC-1 Cells
8:53
Conclusion
Transcripción
To access neural microRNA target interactions with MicroRNA functions is critical to understand how MicroRNAs regulate various biological processes in both healthy and diseased states. This protocol describes the reproduce through strategy to iden
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This protocol uses a probe-based real-time polymerase chain reaction (PCR), a sulforhodamine B (SRB) assay, 3’ untranslated regions (3’ UTR) cloning, and a luciferase assay to verify the target genes of a miRNA of interest and to understand the functions of miRNAs.