Human PC-3 and OVCAR-3 Cancer Cell Pellet Preparation
2:58
High Resolution X-Ray Absorption Spectroscopy (HERFD-XAS) Measuring Procedure
3:40
Results: Selenium (Se) and Iron (Fe) K-Edge Typical HERFD-XANES Analyses
4:56
Conclusion
Transcripción
This protocol has been standardized for cellular specimens, allowing the comparison of experiments running at different synchrotron locations. The main advantage of this technique is a simple and straightforward workflow that allows a fast and rel
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This protocol presents a detailed procedure to prepare biological cryosamples for synchrotron-based X-ray absorption spectroscopy experiments. We describe all the steps required to optimize sample preparation and cryopreservation with examples of the protocol with cancer and phytoplankton cells. This method provides a universal standard of sample cryo-preparation.